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Forkhead Box Q1(FOXQ1)是FOX家族的重要成员之一,在许多肿瘤中异常高表达,而FOXQ1在肝癌中的研究甚少。本研究通过重组慢病毒载体介导的FOXQ1 shRNA感染肝癌SMMC-7721细胞,敲减FOXQ1的表达,研究FOXQ1对SMMC-7721细胞增殖的影响。CCK8法、倍增时间及集落形成实验显示,敲减FOXQ1导致细胞生长减慢,倍增时间延长,细胞集落形成能力减弱。流式细胞技术检测证明,与对照比较,敲减FOXQ1的表达可显著增加G1期细胞、减少S期细胞,提示G1期阻滞。qRT-PCR和Western印迹法显示,cyclin D1和c-Myc表达下调,其可能与G1阻滞有关。上述结果提示,沉默FOXQ1的表达能够抑制SMMC-7721细胞增殖,其机制可能与cyclin D1和c-Myc的下调有关。
Forkhead Box Q1 (FOXQ1) is an important member of the FOX family and is highly expressed in many tumors. FOXQ1 is rarely studied in liver cancer. In this study, FOXQ1shRNA was transfected into hepatocellular carcinoma SMMC-7721 cells by knockdown of FOXQ1 shRNA to knock down the expression of FOXQ1 and study the effect of FOXQ1 on the proliferation of SMMC-7721 cells. CCK8 assay, doubling time and colony formation assay showed that knockdown of FOXQ1 resulted in slowing of cell growth, prolongation of doubling time and decrease of colony forming ability. Flow cytometry showed that compared with the control, knockdown of FOXQ1 expression significantly increased G1 phase cells and decreased S phase cells, suggesting that G1 phase arrest. qRT-PCR and Western blotting showed that cyclin D1 and c-Myc were down-regulated, which may be related to G1 arrest. These results suggest that silencing FOXQ1 expression can inhibit SMMC-7721 cell proliferation, the mechanism may be related to the down-regulation of cyclin D1 and c-Myc.