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MYB转录因子是植物最大的转录因子家族之一,广泛参与植物各种生理生化过程。本研究根据苦荞花期转录组数据,筛选得到一条与拟南芥At MYB1同源性高的序列,采用RT-PCR技术克隆得到该基因,命名为Ft MYB21。生物信息分析表明,该基因编码蛋白含364个氨基酸,相对理论分子量40.0 k D,理论等电点p I 5.79。多重序列比对表明,其编码蛋白属于典型的R2R3型MYB转录因子。进化树分析表明,Ft MYB21蛋白与拟南芥参与抗逆的At MYB1聚为一簇。荧光定量PCR分析表明,Ft MYB21在高盐胁迫下表达量持续显著上升,72 h时达最大值,为对照组的3.35倍;在PEG干旱胁迫下该基因表达量迅速上调,6 h后趋于稳定,为对照组的1.8倍。因此,Ft MYB21基因可能参与苦荞抗高盐和干旱等非生物胁迫的应答反应,这为进一步理解苦荞的抗逆生理奠定了基础。
MYB transcription factor is one of the largest families of transcription factors in plants and is widely involved in various physiological and biochemical processes of plants. In this study, a sequence homologous to Arabidopsis thaliana At MYB1 was screened based on the transcriptome data of the flowering stage of buckwheat. The gene was cloned by RT-PCR and named Ft MYB21. Bioinformatics analysis showed that this gene contained 364 amino acids, with a relative theoretical molecular weight of 40.0 kD and a theoretical isoelectric point of pI5.79. Multiple sequence alignment showed that the encoded protein belonged to typical R2R3 MYB transcription factor. Phylogenetic tree analysis showed that Ft MYB21 protein clustered with Arabidopsis thaliana At MYB1, which is involved in resistance. Quantitative real-time PCR analysis showed that the expression level of MYB21 in Ft MYB21 increased significantly at high salt stress and reached the maximum at 72 h, which was 3.35 times of that in control group. The expression of MYB21 was rapidly up-regulated under PEG drought stress and reached its peak at 6 h Stable, 1.8 times that of the control group. Therefore, Ft MYB21 gene may be involved in the response of tartary buckwheat to abiotic stress such as high salt and drought, which lays the foundation for further understanding of the antiphlogistic physiology of tartary buckwheat.