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目的:通过对大鼠气管内滴注不同浓度的颗粒物质,观察糖尿病大鼠相关炎性因子的改变。方法:将60只6周龄Wister雄性大鼠随机分成正常组(30只)和2型糖尿病模型组(30只),每组随机分为3个小组,即0.9%NaCl组、颗粒物质低剂量组(7.5mg/kg)、颗粒物质高剂量组(30mg/kg),采用气管内滴注法染毒,每个小组再随机分为两组,分别于24及72h后处死大鼠,10%水合氯醛腹腔麻醉,股动脉放血收集血液,检测白细胞、hs-CRP、IL-10、NOS和脂联素水平,取大鼠心肌及颈动脉,HE染色观察血管内皮损伤及心肌组织中性粒细胞浸润情况。结果:各染毒组大鼠血液白细胞、hs-CRP、IL-10、NOS、脂联素水平与0.9%NaCl组比较有显著差异(P<0.05);且颗粒物质高剂量组要明显高于低剂量组(P<0.05);糖尿病模型组以上指标比正常组大鼠组增加更明显(均P<0.05)。结论:大鼠气管内滴入不同浓度的PM2.5后,系统会产生炎症反应,并且随颗粒物质浓度的增高,炎症反应更明显。颗粒物质对糖尿病大鼠心血管系统的影响更加严重,且可能会加速糖尿病的进展。
Objective: To observe the changes of related inflammatory factors in diabetic rats by tracheal instillation of different concentrations of particulate matter. Methods: Sixty Wister male rats aged 6 weeks were randomly divided into normal group (n = 30) and type 2 diabetic model group (n = 30), and each group was randomly divided into three groups: 0.9% NaCl group, low dose of particulate matter Group (7.5mg / kg) and high dose group (30mg / kg). The rats in each group were randomly divided into two groups. Rats were sacrificed at 24 and 72 hours, and 10% Chloral hydrate intraperitoneal anesthesia, blood collection femoral artery blood collection, detection of leukocytes, hs-CRP, IL-10, NOS and adiponectin level, take the rat myocardium and carotid artery, HE staining to observe vascular endothelial injury and myocardial tissue neutrophil Cell infiltration. Results: Compared with 0.9% NaCl group, the level of blood leucocyte, hs-CRP, IL-10, NOS and adiponectin in rats in each exposure group were significantly different (P <0.05) Low-dose group (P <0.05). The above indexes in diabetic model group increased more significantly than those in normal group (all P <0.05). CONCLUSIONS: Instillation of different concentrations of PM2.5 into the trachea of rats results in an inflammatory reaction and the inflammatory response is more pronounced with increasing particulate matter concentration. The effects of particulate matter on the cardiovascular system of diabetic rats are more serious and may accelerate the progression of diabetes.