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目的:研究黄芪甲苷对球囊损伤后大鼠颈动脉内膜增生的影响。方法:SD雄性大鼠50只,随机分为假手术组、模型组、黄芪甲苷(20mg·kg-1·d-1)、(40mg·kg-1·d-1)及(60mg·kg-1·d-1)组。建立大鼠颈动脉球囊损伤模型,黄芪甲苷组大鼠术前3d开始给药,每天1次,假手术组、模型组同时给予1%羧甲基纤维素钠,共给药17d。术后第15dHE染色观察血管内膜的形态学变化;免疫组织化学法检测新生内膜增殖细胞核抗原(PCNA)的表达;比色法检测大鼠血浆超氧化物歧化酶(SOD)、丙二醛(MDA)含量。结果:黄芪甲苷低、中、高剂量组均能明显减少球囊损伤后新生内膜面积、内膜/中膜比(P<0.05),并使PCNA蛋白表达降低(P<0.05);升高球囊损伤后大鼠血浆SOD含量(P<0.05),降低血浆MDA水平(P<0.05)。结论:黄芪甲苷能够抑制大鼠颈动脉球囊损伤后的内膜增生,其主要是通过抑制血管平滑肌的移行、增殖,抗氧化应激反应发挥作用。
Objective: To study the effect of Astragaloside IV on carotid intima hyperplasia after balloon injury in rats. Methods: Fifty SD male rats were randomly divided into sham operation group, model group, astragaloside (20 mg · kg -1 · d -1), (40 mg · kg -1 · d -1) and (60 mg · kg -1) -1 · d-1) group. The model of carotid artery balloon injury was established in rats. The rats in Astragaloside IV group were administered once a day before operation. The rats in sham operation group and model group were given 1% sodium carboxymethyl cellulose simultaneously for 17 days. The morphological changes of vascular intima were observed on the 15th day after operation. The expression of proliferating cell nuclear antigen (PCNA) in neointima was detected by immunohistochemistry. The levels of plasma superoxide dismutase (SOD), malondialdehyde (MDA) content. Results: Astragaloside group could significantly reduce the neointimal area, intima / media ratio (P <0.05) and decrease the expression of PCNA protein (P <0.05) after balloon injury in low, middle and high dose groups; After balloon injury, the content of SOD in plasma of rats decreased (P <0.05), and the level of plasma MDA decreased (P <0.05). CONCLUSION: Astragaloside can inhibit intimal hyperplasia after carotid artery balloon injury in rats. Astragaloside mainly plays a role in inhibiting the migration, proliferation and anti-oxidative stress of vascular smooth muscle.