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将受孕的雌鼠随机分为低、中、高3个染毒剂量组和溶剂对照组,每组15只孕鼠。分别染毒1、4、8 mg/kg的甲胺基阿维菌素苯甲酸盐原药,溶剂对照组经口给予蒸馏水。对照组和各染毒组在母鼠受孕后7～16 d,采用灌胃法染毒,于妊娠第20天处死母鼠,观察母鼠和胎鼠的生长发育情况。8 mg/kg组妊娠动物于染毒3～5 d后出现间断性四肢颤抖和抽搐,染毒结束后,症状逐渐减轻或消失;胎鼠的骨骼检查中可见枕骨发育不全和胸骨缺失,骨骼畸形率为12.76%,与溶剂对照组比较差异有统计学意义(P<0.01)。中、低剂量组各项检查与对照组比较差异无统计学意义。提示在本实验条件下,甲胺基阿维菌素苯甲酸盐原药对大鼠的母体毒性和胚胎发育毒性的未观察到有害作用剂量(NOA-EL)为4 mg/kg;无致畸作用。
The conceived female rats were randomly divided into low, medium and high exposure dose groups and solvent control group, each 15 pregnant rats. Respectively, 1, 4, 8 mg / kg of methotericin benzoate original drug, solvent control group were given distilled water. In the control group and each exposure group, the mother rats were sacrificed 7-16 days after conception. The rats were sacrificed on the 20th day of gestation to observe the growth and development of the mother rats and the fetus. In the 8 mg / kg group, intermittent limb extremities tremor and convulsions occurred in gestational animals 3 to 5 days after exposure. The symptom gradually lightened or disappeared after the exposure of the pregnant animals. Fetal aplasia and sternum loss, skeletal deformity The rate was 12.76%, compared with the solvent control group, the difference was statistically significant (P <0.01). There was no significant difference between the low-dose group and the control group. It is suggested that the NOA-EL of 4 mg / kg on the maternal toxicity and embryo developmental toxicity of emamectin benzoate in this experimental condition is not observed. Teratogenic effect.