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用从微球菌中提取纯化的过氧化氢酶为免疫原，以硝酸纤维膜皮下埋植法免疫ＢＡＬＢ／ｃ小鼠，取其脾细胞和ＳＰ２／０骨髓瘤细胞融合，获得１１株能稳定分泌抗体的杂交瘤细胞系。其中１Ａ１０、３Ｃ５、４Ｅ１２和４Ｆ６四株经连续传代三个月、冻存１０个月后复苏，仍能稳定分泌抗体．腹水效价在１０－４～１０－６之间．Ｉｇ类型鉴定，１Ａ１０与４Ｅ１２为ＩｇＧ２ａ，４Ｆ６为ＩｇＧ３亚类，３Ｃ５为ＩｇＭ类．染色体数介于８２～１１０之间．将４株ＭｃＡｂ与溶菌酶、纤维结合蛋白等１１种蛋白及多肽物质做交叉反应试验，均无明显交叉反应．以固相抗原竞争ＥＬＩＳＡ试验和ＥＬＩＳＡ相加试验作位点分析，两法一致表明ＭｃＡｂ４Ｆ６与另３株ＭｃＡｂ１Ａ１０、３Ｃ５和４Ｅ１２所针对的抗原位点有差别。４株ＭｃＡｂ相对亲和力测定结果依次为：４Ｅ１２＞１Ａ１０＞３Ｃ５＞４Ｆ６．用亲和层析柱纯化的４Ｆ６ＭｃＡｂ做过氧化氢酶催化活性试验，发现能激活过氧化氢酶对底物索曼的活性，抑制过氧化氢酶对底物过氧化氢的分解．
BALB / c mice were immunized with nitrocellulose membrane subcutaneously by using purified catalase extracted from Micrococci as immunogen. The spleen cells and SP2 / 0 myeloma cells were fused to obtain 11 strains that could stably secreted Antibody hybridoma cell line. Among them, four strains of 1A10, 3C5, 4E12 and 4F6 were passaged for three months in succession and resumed after 10 months of cryopreservation, which could still stably secrete antibodies. Ascites potency between 10-4 ~ 10-6. Ig type identification, 1A10 and 4E12 for IgG2a, 4F6 for IgG3 subclass, 3C5 for IgM class. Chromosome number between 82 ~ 110. Four McAbs were tested for their cross-reactivity with 11 proteins and peptides, such as lysozyme and fibronectin, without significant cross-reaction. The solid phase antigen competition ELISA test and ELISA additive test for site analysis, two methods consistently show that the McAb4F6 and the other three McAb1A10, 3C5 and 4E12 against the antigenic site differences. The relative affinities of 4 McAbs were 4E12> 1A10> 3C5> 4F6. The 4F6McAb purified by affinity chromatography column was used to test the activity of catalase. It was found that catalase could activate catalase activity of substrate catalase and inhibit catalase decomposition of substrate hydrogen peroxide.