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本实验对胚脑组织进行了冷冻保存、培养和移植，以观察冻存后的胚脑组织在体外及宿主脑内继续生长、分化的情况。结果显示：培养４～５小时后细胞贴壁，１天后开始有轴突长出，在所有贴壁细胞中，有５０％～５８％能继续经培养后存活。把经冻存的胚脑细胞移植到幼鼠脑内，４０～４５天后尼氏染色切片观察，细胞形态正常，与周围宿主脑区有明显的界限。本实验证实冻存的胚脑组织复苏后仍保持继续生长、分化和建立突触联系的特性。
In this experiment, embryonic brain tissues were cryopreserved, cultured and transplanted to observe the continued growth and differentiation of embryonic brain tissue in vitro and in the host brain after cryopreservation. The results showed that the cells adherent to the wall after 4 to 5 hours of culture, and the axons started to grow after 1 day. In all the adherent cells, 50% to 58% of the adherent cells could continue to survive after culturing. The frozen embryonic brain cells transplanted into the brain of young rats, 40 to 45 days after Nissl staining section, normal cell morphology, and the surrounding host brain area with clear boundaries. This experiment confirmed that frozen embryonic brain tissue after the resumption of continued to maintain growth, differentiation and the establishment of the characteristics of synaptic connections.