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目的制备和厚朴酚脂质体(HK-Lipsomes),探讨其对小鼠乳腺癌细胞(4T1细胞)的体内外生长抑制作用。方法将蛋黄卵磷脂、胆固醇、m PEG2000-DSPE2000、和厚朴酚按照质量比3:1:1:1,以注入法制备成和厚朴酚脂质体。通过Zetasizer nano ZS测定和厚朴酚脂质体的粒径,透射电镜观察和厚朴酚脂质体的形态。用噻唑蓝(MTT)比色法评价和厚朴酚脂质体对4T1细胞的细胞毒性。建立小鼠4T1乳腺癌肿瘤模型,以紫杉醇注射液为阳性对照,考察腹腔给药后和厚朴酚脂质体对肿瘤的抑制作用。结果制备得到的和厚朴酚脂质体的平均粒径为(113.8±0.2)nm,多分散指数(0.209±0.005),电位为(-30.7±2.4)m V,透射电镜观察和厚朴酚脂质体为球型。和厚朴酚溶液和脂质体对4T1细胞的IC50值分别为(43.74±2.38)μg/m L和(12.52±2.24)μg/m L。和厚朴酚脂质体高(60 mg/kg)、中(40 mg/kg)、低(20 mg/kg)剂量组的抑瘤率分别为85.19%、60.95%和37.83%,呈剂量相关性。结论实验使用较为简便的制备方法成功制备粒径较小、包封率高的和厚朴酚脂质体。荷瘤小鼠体内实验显示抑瘤效果良好。
Objective To prepare honokioliposomes (HK-Lipsomes) and investigate their inhibitory effect on the growth of mouse breast cancer cells (4T1 cells) in vitro and in vivo. Methods Honokiol, cholesterol, m PEG2000-DSPE2000 and honokiol were prepared by injection method according to the mass ratio of 3: 1: 1: 1. The particle size of honokiol liposomes was determined by Zetasizer nano ZS, and the morphology of honokiol liposomes was observed by transmission electron microscope. Cytotoxicity of honokiol liposomes to 4T1 cells was evaluated by MTT colorimetry. The mouse model of 4T1 breast cancer was established. Paclitaxel injection was used as a positive control to observe the inhibitory effect of honokiol liposomes on the tumor after intraperitoneal administration. Results The average particle size of honokiol liposomes was (113.8 ± 0.2) nm, the polydispersity index (0.209 ± 0.005) and the potential were (-30.7 ± 2.4) mV. The results of transmission electron microscopy showed that honokiol Liposomes are spherical. The IC50 values of honokiol solution and liposome to 4T1 cells were (43.74 ± 2.38) μg / m L and (12.52 ± 2.24) μg / m L, respectively. The inhibitory rates of honokiol liposome (60 mg / kg), medium (40 mg / kg) and low (20 mg / kg) were 85.19%, 60.95% and 37.83% . Conclusions The simpler preparation method was used to successfully prepare honokiol liposomes with small size and high encapsulation efficiency. Tumor-bearing mice in vivo experiments showed good anti-tumor effect.