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目的 用 pc- ROP1重组质粒免疫小鼠 ,观察其对细胞因子 IFN-γ、IL - 2及 NO的影响。方法 碱裂解法大量制备 pc- ROP1质粒 DNA,经肌肉注射免疫 BAL B/ c小鼠 ,每只鼠注射 10 0μg,两周后同量加强免疫一次 ,以 pc DNA 3空质粒及生理盐水组为对照。分别于免疫后第 30天、5 0天、70天共三次用双夹心 EL ISA测定血清细胞因子 IFN-γ、IL - 2含量 ;酶法测定NO活性。结果 三次检测免疫组 IFN-γ、IL - 2及 NO含量均显著高于对照组 ,随着免疫时间的延长有增高趋势。结论 pc-ROP1重组质粒 DNA免疫小鼠 ,可刺激细胞因子 IFN-γ、IL - 2产生 ,NO分子的释放
Objective To immunize mice with pc-ROP1 recombinant plasmids to observe the effect on cytokines IFN-γ, IL-2 and NO. Methods Plasmid pc-ROP1 was prepared in large quantities by alkaline lysis. BALB / c mice were immunized with intramuscular injection. Each mouse was injected with 100 μg of recombinant plasmids. After two weeks of immunization with the same amount of plasmid, pcDNA3 null plasmid and normal saline Control. The levels of IFN-γ and IL-2 in serum were measured by double-sandwich ELISA at 30 days, 50 days and 70 days after immunization. The activity of NO was measured by enzymatic method. Results The levels of IFN - γ, IL - 2 and NO in the three immunized groups were significantly higher than those in the control group, and increased with the prolongation of immunization time. Conclusion The pc-ROP1 recombinant plasmid DNA immunized mice could stimulate the production of cytokines IFN-γ, IL-2 and NO release