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目的观察东菱迪夫对脑缺血损伤后大鼠神经干细胞分化、迁移的影响,并探讨东菱迪夫治疗急性脑缺血损伤的可能机制。方法采用大脑中动脉阻塞法(MCAO)建立局灶性脑缺血再灌注模型;运用神经功能缺陷评分和TTC染色评定神经功能与脑梗死体积;免疫组化检测缺血侧脑室下区(SVZ)5-溴脱氧尿嘧啶核苷(BrdU)以及胶质纤维酸性蛋白GFAP(作为检测脑缺血后神经干细胞的分化、迁移的指标),从而评价东菱迪夫对缺血脑损伤后神经干细胞分化迁移的作用。结果东菱迪夫组(DLDF)再灌后第7天脑梗死灶体积明显小于模型组(P<0.01),DLDF组大鼠在第7、8、9、16天的神经功能缺陷评分明显优于模型组(P<0.05);和模型组相比,缺血损伤后第7天,东菱迪夫组大鼠SVZ的GFAP阳性细胞数明显增多(P<0.05);缺血损伤后第14天,东菱迪夫组大鼠SVZ的BrdU阳性细胞数明显增多(P<0.05),并且观察到从SVZ至其背外侧带的BrdU阳性细胞的迁移带。结论东菱迪夫能减少脑缺血大鼠的脑梗死灶体积和改善神经功能评分;缺血脑损伤后,脑内神经干细胞发生分化、迁移;东菱迪夫可促进SVZ神经干细胞的分化、迁移。
Objective To observe the effect of dongluidifu on the differentiation and migration of neural stem cells in rats after cerebral ischemic injury and to explore the possible mechanism of dongluidifu in treating acute cerebral ischemic injury. Methods The focal cerebral ischemia-reperfusion model was established by middle cerebral artery occlusion (MCAO). Neurological deficits and cerebral infarction volume were assessed by neurological deficit scores and TTC staining. Immunohistochemistry was used to detect the expression of SVZ, Bromodeoxyuridine (BrdU) and glial fibrillary acidic protein GFAP (as indicators of differentiation, migration of neural stem cells after cerebral ischemia), so as to evaluate the effect of dongluidifu on neural stem cell differentiation and migration after ischemic brain injury Role. Results The volume of cerebral infarction in DLDF group on the 7th day after reperfusion was significantly lower than that in the model group (P <0.01). The score of neurological deficit in DLDF group on day 7, 8, (P <0.05). Compared with the model group, the number of GFAP positive cells in SVZ of Dongluidifu group was significantly increased on the 7th day after ischemia (P <0.05). On the 14th day after ischemia injury, The number of BrdU positive cells in SVZ of dongluodifu group was significantly increased (P <0.05), and the migration of BrdU positive cells from SVZ to the dorsolateral zone was observed. Conclusion Dongluodifu can reduce cerebral infarction volume and improve neurological function score of cerebral ischemia rats. After ischemic brain injury, neural stem cells of brain differentiate and migrate. Dongluidifu can promote the differentiation and migration of neural stem cells of SVZ.