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目的:探讨荜茇乙醇提取物对哮喘小鼠气道炎症的治疗作用及其作用机制。方法:40只清洁级BABL/c小鼠按随机数字表法分为4组,分别为生理盐水对照组、哮喘模型组、荜茇乙醇提取物低和高剂量组(100 mg/kg、200 mg/kg)。在末次激发24 h后所有小鼠取左肺组织行HE染色,观察哮喘组肺组织炎症浸润情况。用酶联免疫吸附法(ELISA)观察小鼠支气管肺泡灌洗液(BALF)中细胞的总数和各种炎症细胞计数的变化以及白细胞介素-4(IL-4)、IL-5及干扰素-γ(IFN-γ)含量;取右肺组织用Westernblot检测NF-kB p65蛋白的表达情况。结果:哮喘模型组小鼠与对照组相比较BALF中的嗜酸粒细胞计数、IL-4、IL-5水平增高和IFN-γ水平降低,肺组织中的NF-kB p65表达水平显著高于对照组;荜茇乙醇提取物低剂量组(100 mg/kg)和高剂量组(200 mg/kg)小鼠与哮喘组小鼠相比炎症细胞计数、IL-4、IL-5以及NF-kB p65蛋白表达水平均显著降低而IFN-γ水平明显上升,具有显著的差异。结论:荜茇乙醇提取物在体内可能主要通过NF-kB p65等转录因子调节Th2反应。
Objective: To investigate the therapeutic effect and its mechanism of alcohol extract of tragacanth on airway inflammation in asthmatic mice. Methods: Forty clean-grade BABL / c mice were randomly divided into four groups according to random number table: saline control group, asthma model group, ethanol extract of low-and high-dose group (100 mg / kg, 200 mg / kg). The left lungs of all mice were stained with HE 24 hours after the last challenge, and the infiltration of inflammatory cells in the lung tissue of the asthma group was observed. The total number of cells in the bronchoalveolar lavage fluid (BALF) and the changes of various inflammatory cell counts as well as the changes of interleukin-4 (IL-4), IL-5 and interferon γ (IFN-γ) content was measured. Right lung tissue was used to detect the expression of NF-κB p65 protein by Western blot. Results: Compared with the control group, the number of eosinophils, the levels of IL-4, IL-5 and the level of IFN-γ in BALF in asthmatic model group were significantly decreased The levels of IL-4, IL-5 and NF-κB in the mice in the control group were lower than those in the low-dose ethanol extract group (100 mg / kg) and high-dose (200 mg / kB p65 protein expression levels were significantly lower and IFN-γ levels increased significantly, with significant differences. CONCLUSION: The extract of alcohol extract of Quchi may regulate Th2 response mainly through transcription factors such as NF-kB p65 in vivo.