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目的:探讨微弧氧化(micro-arc oxidation,MAO)后的Zr46(Cu4.5/5.5Ag1/5.5)46Al8(at%)(本文简称Zr-Cu-Al-Ag非晶合金)的细胞相容性。方法:按照国家标准制备300 V、350 V和400 V电压MAO处理的Zr-Cu-Al-Ag非晶合金、铸态Zr-Cu-Al-Ag非晶合金以及TI6Al4V合金试件的浸提液用于培养L929细胞,阴性组的L929细胞用含10%小牛血清的DMEM溶液培养,阳性组的L929细胞用含64 g/L苯酚和10%小牛血清的DMEM溶液培养,通过四唑盐(MTT)比色法分析试件的细胞相容性。结果:MAO处理的Zr-Cu-Al-Ag非晶合金细胞毒性评级为0,其浸提液中的L929细胞状态良好,细胞增殖曲线呈上升趋势,三个MAO组的吸光度值高于铸态Zr-Cu-Al-Ag非晶合金组、TI6Al4V合金组和阳性对照组(P<0.05),但与阴性对照组无明显差别(P>0.05)。结论:MAO提高了Zr-Cu-Al-Ag非晶合金表面的细胞相容性。
Objective: To investigate the cytocompatibility of Zr46 (Cu4.5 / 5.5Ag1 / 5.5) 46Al8 (at%) (abbreviated as Zr-Cu-Al-Ag amorphous alloy) after micro-arc oxidation (MAO) Sex. Methods: The preparation of Zr-Cu-Al-Ag amorphous alloy, as-cast Zr-Cu-Al-Ag amorphous alloy and TI6Al4V alloy specimen at 300 V, 350 V and 400 V according to the national standard For the culture of L929 cells, L929 cells in the negative group were cultured in DMEM containing 10% fetal bovine serum. L929 cells in the positive group were cultured in DMEM solution containing 64 g / L phenol and 10% fetal bovine serum, (MTT) colorimetric assay of the cell compatibility. Results: The cytotoxicity of MAO-treated Zr-Cu-Al-Ag amorphous alloy was 0, the L929 cells in the extract were in good condition and the cell proliferation curve showed an upward trend. The absorbance values of the three MAO groups were higher than those in the as-cast Zr-Cu-Al-Ag amorphous alloy group, TI6Al4V alloy group and positive control group (P <0.05), but no significant difference with the negative control group (P> 0.05). Conclusion: MAO improves the cytocompatibility on the surface of Zr-Cu-Al-Ag amorphous alloy.