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[Objective] This study aimed to explore a modified method for improving the efficiency of RAPD technology.[Method] Longans collected from Sichuan,Fujian,Guangxi,Guangdong and Hainan were used as experimental materials.Total genomic DNA was extracted from longan leaves with modified CTAB method.18 random primers were selected for RAPD amplification with ramp rates of 0.3 and 3.0 ℃/s,respectively.Effects of different ramp rates on RAPD amplification were compared and the relationship among longan samples was analyzed.[Result] The resolution and yield could be improved significantly by declining the ramp rate from 3.0 to 0.3 ℃/s.Cluster analysis showed that similarity coefficient of the five varieties of longan ranged from 0.69 to 0.76.[Conclusion] This study had confirmed that reducing ramp rate could significantly increase the resolution and yield of RAPD technology,which had provided a new method to improve the efficiency of RAPD.
[Objective] This study aimed to explore a modified method for improving the efficiency of RAPD technology. [Method] Longans collected from Sichuan, Fujian, Guangxi, Guangdong and Hainan were used as experimental materials. Total genomic DNA was extracted from longan leaves with modified CTAB method.18 random primers were selected for RAPD amplification with ramp rates of 0.3 and 3.0 ° C / s, respectively. Effects of different ramp rates on RAPD amplification were compared and the relationship among longan samples was analyzed. [Result] The resolution and yield could be improved significantly by declining the ramp rate from 3.0 to 0.3 ° C / s. Cluster analysis showed that similarity coefficient of the five varieties of long ranged from 0.69 to 0.76. [Conclusion] This study has confirmed that reducing ramp rate could significantly increase the resolution and yield of RAPD technology, which had provided a new method to improve the efficiency of RAPD.