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c-myc的扩增为引发肿瘤的关键因素之一。为探讨丝裂霉素C(mitomycin MMC)和小牛血清对c-myc扩增的影响。本文以基因转移方法,将c-myc导入NIH/3T3细胞,并分别加入不同浓度MMC和5%小牛血清于转染后NIH/3T3(After transfectionNIH/3T3 AN)细胞,经点杂交检测细胞内c-myc的含量。
Amplification of c-myc is one of the key factors for tumor initiation. To investigate the effect of mitomycin MMC and calf serum on c-myc amplification. In this study, c-myc was introduced into NIH/3T3 cells by gene transfer method, and NIH/3T3 (After transfection NIH/3T3 AN) cells were transfected with different concentrations of MMC and 5% fetal calf serum and detected by spot hybridization. C-myc content.