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作者用pBMGneo质粒的HCMC启动子置换pSBC-1和PSBC-2质粒中的SV40启动子得到pCMV-1和pCMV-2表达质粒.在PCMV-1中插入含有HBcAg基因的pBl-uescript IISK+酶切片段得到能表达 HBcAg的单顺反子质粒pCMV-1/C;同样在pCMV-2中插入含有HBsAg基因的pTKT HBV2片段得到能表达HBsAg的单顺反子质粒pCMV-2/S.连接 pCMV-1地和 pCMV-2后质粒的Mstl/Notl酶切片段则构成含HBcAg和HBsAg抗原基因的双顺反子表达质粒pCMV/C-S.
The pCMV-1 and pCMV-2 expression plasmids were obtained by replacing the SV40 promoter in pSBC-1 and PSBC-2 plasmids with the HCM promoter of pBMGneo plasmid.The pBl-uescript IISK + digested fragment containing HBcAg gene To obtain a monocistronic plasmid pCMV-1 / C capable of expressing HBcAg; a monocistronic plasmid pCMV-2 / S capable of expressing HBsAg was also inserted into the pTKT HBV2 fragment containing the HBsAg gene in pCMV-2. The Mstl / Notl fragment from pCMV-2 and pCMV-2 plasmids formed the bicistronic expression plasmid pCMV / CS containing the HBcAg and HBsAg antigens.