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本文报道了利用β-羟基丁酸脱氢酶催化反应来测定血浆中β-羟基丁酸(β-OHBA)与乙酰乙酸(AcAc)的含量。由于采用荧光分光光度法,测定的灵敏度、精确度、回收率与重复性均较理想。糖尿病患者血浆β- OHBA与AcAc的含量明显高于正常人。在糖尿病患者中,Ⅰ型病例的含量又明显高于Ⅱ型,而酮症酸中毒患者的含量更远高于Ⅰ型病例。因此β-OHBA与AcAc的测定对于分析糖尿病的代谢障碍和酮症的早期诊断与随访均具有价值。
In this paper, β-hydroxybutyrate (β-OHBA) and acetoacetate (AcAc) contents in plasma were determined by the use of β-hydroxybutyrate dehydrogenase. As a result of fluorescence spectrophotometry, determination of the sensitivity, accuracy, recovery and repeatability are more ideal. The plasma levels of β-OHBA and AcAc in diabetic patients were significantly higher than those in normal subjects. In patients with type 1 diabetes mellitus, the content of type I was significantly higher than that of type II, while those with ketoacidosis were much higher than type I. Therefore, the determination of β-OHBA and AcAc is valuable for the early diagnosis and follow-up of metabolic disorders and ketosis for the diagnosis of diabetes.