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目的:探讨伸筋草、车前子、秦皮、络石藤等中药组方(简称伸秦组方)对高尿酸血症小鼠的影响及作用机制。方法:采取联合使用腺嘌呤、乙胺丁醇、酵母膏连续灌胃给药,制备小鼠高尿酸血症模型。同时给予西药和中药伸秦组方进行对照,26天后检测各组小鼠的血清尿酸值和黄嘌呤氧化酶(XOD)、腺苷脱氨酶(ADA)的活性;并取肾脏做组织切片,在光镜下观察其组织变化。结果:伸秦组方能降低小鼠血尿酸(与模型组比,P<0.01),在抑制ADA和XOD活性方面优于其余各组(P<0.01)。中药组小鼠肾脏中尿酸结晶较西药组少,肾脏损害较小。结论:伸秦组方有治疗高尿酸血症和良好的肾脏保护作用。
Objective: To investigate the effects of Shenjincao, Chehsicazi, Qinpi, Luoshiteng and other herbal prescriptions (abbreviated as Shenqin prescription) on hyperuricemia in mice and its mechanism of action. Methods: The model of mouse hyperuricemia was prepared by continuous intragastric administration of adenine, ethambutol and yeast extract. At the same time, western medicine and traditional Chinese medicine Shenqin group were given to control. After 26 days, serum uric acid, xanthine oxidase (XOD) and adenosine deaminase (ADA) activity were measured in each group of mice; kidneys were taken for tissue sectioning. Observe the changes in the tissue under light microscope. Results: Shenqin group can reduce the serum uric acid in mice (P<0.01 compared with the model group), and is superior to other groups in inhibiting the activity of ADA and XOD (P<0.01). The uric acid crystals in the kidneys of mice in the traditional Chinese medicine group were less than those in the western medicine group, and renal damage was small. Conclusion: Shenqin group can treat hyperuricemia and have good renal protection.