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目的:测定小鼠血浆中的盐酸小檗碱,以考察连香微乳在小鼠体内的生物利用度。方法:采用小鼠单次灌胃给药,在不同时间点取血,建立高效液相色谱法测定盐酸小檗碱的血药浓度;以连香普通乳为参比,计算连香微乳的相对生物利用度。结果:盐酸小檗碱与血浆中内源性杂质分离度好,线性范围为10.4～156μg/L(r=0.9991),方法回收率在89.6%～94.7%之间,日内和日间精密度RSD≤12.9%;单次灌胃给药后连香微乳和连香普通乳中盐酸小檗碱的AUC0→24 h分别为(688.3±123.7)、(371.4±68.4)μg.h/L;连香微乳相对于连香普通乳的生物利用度为(193.1±63.2)%。结论:建立的高效液相色谱法可用于测定小鼠血浆中的盐酸小檗碱;微乳化可显著提高连香方药中盐酸小檗碱在小鼠体内的生物利用度。
Objective: To determine the berberine hydrochloride in mouse plasma to investigate the bioavailability of Lianxiang microemulsion in mice. METHODS: Mice were given a single intragastric administration and blood was taken at different time points to establish a high performance liquid chromatography method for determination of berberine hydrochloride blood concentration. Using Lianxiang Ordinary Milk as a reference, a calculation was made of the Lianxiang microemulsion. Relative bioavailability. RESULTS: The separation of berberine hydrochloride and endogenous impurities in plasma was good, with a linear range of 10.4 to 156 μg/L (r=0.9991). The method recoveries ranged from 89.6% to 94.7%. The intra-day and inter-day precision RSD ≤12.9%; AUC0→24 h of berberine hydrochloride in Lianxiang microemulsion and Lianxiang Normal Milk after single oral administration was (688.3±123.7) and (371.4±68.4) μg.h/L, respectively; The bioavailability of fragrant microemulsions relative to Lianxiang Normal Milk was (193.1±63.2)%. Conclusion: The established HPLC method can be used to determine berberine hydrochloride in mouse plasma. Microemulsification can significantly increase the bioavailability of berberine hydrochloride in mice in Lianxiang Recipe.