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目的:研究Th17细胞及相关因子白细胞介素17(IL-17)在小鼠肾移植排斥反应中的表达及意义。方法:建立小鼠肾移植模型,实验动物随机分为同系移植组和急性排斥反应组;在移植术后3,7 d分别应用ELISA检测2组小鼠血清中IFN-γ和IL-17的含量,应用流式细胞技术检测移植肾中浸润淋巴细胞中Th1和Th17细胞数量,取移植肾经10%甲醛固定后行常规病理检查。结果:与同系移植组相比,急性排斥反应组术后第3天血清IFN-γ含量无明显差异而IL-17含量显著增高(P<0.05),术后第7天血清IFN-γ和IL-17含量均显著增高(P<0.05);急性排斥反应组中血清IFN-γ和IL-17含量术后第7天较第3天均显著增高(P<0.05);急性排斥反应组移植肾中浸润淋巴细胞中Th1与Th17细胞比例在术后第3天及第7天较同系移植组均明显增多(P<0.05);急性排斥反应组中移植肾中浸润淋巴细胞中Th1与Th17细胞比例术后第7天明显高于第3天(P<0.05);病理组织学检查急性排斥反应组随着移植时间的延长,排斥反应逐渐增强。结论:Th17细胞在肾移植排斥反应的发生发展中可能起着非常重要的作用,对受体血清中细胞因子IL-17的检测可作为急性排斥反应早期诊断的预见性和特异性指标。
Objective: To investigate the expression and significance of Th17 cells and related factors interleukin-17 (IL-17) in renal allograft rejection in mice. Methods: The model of kidney transplantation in mice was established. The experimental animals were randomly divided into three groups: the homograft group and the acute rejection group. The levels of IFN-γ and IL-17 in serum of the two groups were detected by ELISA at 3 and 7 days after transplantation Flow cytometry was used to detect the numbers of Th1 and Th17 cells in infiltrating lymphocytes of allograft recipients. The grafts were fixed with 10% formalin and routinely examined. Results: Compared with the homograft group, the levels of serum IFN-γ and IL-17 in the acute rejection group were significantly increased (P <0.05) on the third day after operation. The levels of IFN-γ and IL-7 (P <0.05). The levels of serum IFN-γ and IL-17 in acute rejection group were significantly higher than those on the 3rd day after operation (P <0.05), while those in the acute rejection group were higher than those in the control group The proportion of Th1 and Th17 cells in infiltrating lymphocytes in the group of infiltrating lymphocytes increased significantly (P <0.05) on the 3rd day and the 7th day after transplantation compared with those in the same line transplantation group (P <0.05). The proportion of Th1 and Th17 cells in the infiltrating lymphocytes in the acute rejection group On the 7th postoperative day, the rejection rate was significantly higher than that on the third day (P <0.05). The pathological examination showed that the rejection increased with the prolongation of transplantation time in the acute rejection group. CONCLUSION: Th17 cells may play an important role in the development of renal allograft rejection. Detection of IL-17, a cytokine in recipient serum, may serve as a predictive and specific indicator for the early diagnosis of acute rejection.