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用密度梯度离心和贴壁法分离和纯化兔骨髓间充干细胞 ,建立诱导兔 MSCs向脂肪细胞及成骨细胞表型转化的方法及条件。在成脂诱导剂或成骨诱导剂作用下 ,对原代和第 2代兔 MSCs进行成脂和成骨诱导培养 ,并鉴定成脂及成骨表型。结果表明 :原代及第 2代兔 MSCs均有一定的成脂、成骨能力 ,且第 2代细胞的分化能力较原代低。在诱导培养条件下 ,原代及第 2代兔 MSCs均能分化 ,成脂诱导 2 1d,75 %的兔 MSCs转化为脂肪细胞 ;成骨诱导 2 1d,75 %的兔 MSCs转化为成骨细胞。兔 MSCs在适当的诱导条件下可快速分化为脂肪细胞或成骨细胞
Methods The separation and purification of rabbit bone marrow stem cells by density gradient centrifugation and adherent method were used to establish the method and conditions for inducing the MSCs to transform into phenotype of adipocytes and osteoblasts. Under the action of adipogenic or osteogenic agents, the primary and second generation of rabbit MSCs were induced to adipogenesis and osteogenesis, and the adipogenic and osteogenic phenotypes were identified. The results showed that both primary and secondary rabbit MSCs had some adipogenic and osteogenic potential, and the second generation of cells had lower differentiation ability than the primary ones. Under induction culture conditions, both primary and secondary rabbit MSCs could differentiate into adipocytes induced by adipogenic 21d and 75% of rabbit MSCs. On the 21st day after osteogenic induction, 75% of MSCs were transformed into osteoblasts . Rabbit MSCs can rapidly differentiate into adipocytes or osteoblasts under appropriate inducing conditions