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[目的]对梅毒螺旋体抗体胶体金法检测血清抗梅毒螺旋体抗体阳性的标本,同时用TRUST法、ELISA法和TPPA法进行检测,以探讨阳性结果是否存在方法学导致的假阳性。[方法]11 200例无梅毒症状的住院及门诊普通病人为实验组,210例性病门诊病人为对照组。用胶体金进行抗梅毒抗体筛查,检测阳性的标本再用酶联免疫吸附试验(ELISA),甲苯胺红不加热血清试验(TRUST)和梅毒螺旋体明胶颗粒凝集试验(TPPA)进行检测,以TPPA法为标准对检测结果进行对比分析。[结果]实验组中检出胶体金法阳性181例,经TPPA法检测确认阳性126例,阳性符合率为69.6%(126/181);以ELISA法检出阳性124例,经TPPA确认全为阳性,阳性符合率为98.4%(124/126);TRUST法检出阳性100例,经TPPA确认阳性91例,9例为阴性;3种方法即ELISA,金标法,TRUST法均阳性为89例。对照组中胶体金法阳性80例,TPPA法阳性51例,ELISA法阳性51例,TRUST法阳性40例,3种方法即ELISA,金标法,TRUST法均阳性为39例。[结论]ELISA法测定抗梅毒抗体与TPPA法有较高的阳性符合率;对TRUST的结果应综合分析;如果多种方法联合检测,可使假阳性率下降。
[Objective] To detect the positive anti-Treponema pallidum antibody by colloidal gold assay of Treponema pallidum antibody, and to test the positive results by TRUST, ELISA and TPPA methods. [Method] 11 200 common inpatients and outpatients without syphilis symptoms were experimental group and 210 STD outpatients were control group. The anti-syphilis antibodies were screened with colloidal gold, and the positive samples were detected by enzyme-linked immunosorbent assay (ELISA), TRUST and TPPA, and TPPA France as the standard test results for comparative analysis. [Results] In the experimental group, 181 cases were positive by colloidal gold assay, 126 cases were positive by TPPA assay, the positive coincidence rate was 69.6% (126/181); 124 cases were positive by ELISA assay and all were confirmed by TPPA The positive rate was 98.4% (124/126). The positive rate of TRUST was 100, the positive rate of TPPA was 91, and the negative rate of 9 was negative. The positive rates of ELISA, gold standard and TRUST were 89 example. In control group, 80 cases were positive for colloidal gold, 51 cases were positive for TPPA, 51 cases were positive for ELISA and 40 cases were positive for TRUST. There were 39 cases positive for ELISA, gold standard and TRUST. [Conclusion] The ELISA test shows that the anti-syphilis antibody has a high positive coincidence rate with TPPA method. The results of TRUST should be comprehensively analyzed. If the combination of multiple methods is used, the false-positive rate can be decreased.