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目的 用大鼠大脑中动脉梗塞模型评价血红蛋白近红外光谱 (NIRS)变化特征 ,实现对大脑梗塞灶的定位诊断。方法 计算并搭建了双波长 (85 0nm和 76 0nm)NIRS拓扑系统。用 2 0只SD大鼠建立大脑中动脉梗塞模型 ,并对梗塞灶成像 ;然后用磁共振 (MRI)和化学染色方法对同一只大鼠梗塞灶成像 ,并比较不同技术显示的梗塞灶位置和面积。结果 重建的NIRS拓扑图显示大鼠大脑左侧皮层有一低的光密度区 ,平均梗塞面积为 19 5 0mm2 (19 5 0± 0 35mm2 ,n =2 0 ) ;染色的解剖样品显示平均梗塞面积为 18 4 6mm2 (18 4 6± 0 38mm2 ,n =2 0 ) ;MRI显示平均梗塞面积为 2 0 71mm2 (2 0 71± 0 2 7mm2 ,n =2 0 ) ,三种技术显示的平均梗塞面积无显著性差异 (F(2 ,5 7) =2 4 7,P >0 0 5 )。NIRS与MRI、解剖样品之间相关系数分别为 (r=0 82 ,P <0 0 5 )、(r =0 89,P <0 0 1)。同时 ,NIRS拓扑图显示的梗塞灶的位置和几何形状也为同一只大鼠的MRI、解剖样品所证实。结论 研究结果提示 ,NIRS可以实时跟踪各种因素引起的大脑皮层血流变化。
Objective To evaluate the changes of hemoglobin near infrared spectroscopy (NIRS) using a model of middle cerebral artery occlusion in rats, and to realize the localization diagnosis of cerebral infarction. Methods Two wavelength (85 0nm and 76 0nm) NIRS topologies were calculated and constructed. The model of middle cerebral artery occlusion (MCAO) was established with 20 SD rats, and the infarct was imaged. Then the infarct of the same rat was imaged by MRI and chemical staining, and the infarct location showed by different techniques area. RESULTS: The reconstructed NIRS topography showed a low optical density region in the left cortex of the rat brain with an average infarct size of 195 mm2 (19500 ± 0.35 mm2, n = 20). The stained anatomical samples showed an average infarct size of 18 4 6mm2 (18 4 6 ± 0 38mm2, n = 20). MRI showed an average infarct size of 2 0 71mm2 (2 0 71 ± 0 2 7mm2, n = 2 0). The average infarct size Significant difference (F (2, 5 7) = 2 4 7, P> 0 0 5). The correlation coefficients between NIRS and MRI and anatomic samples were (r = 0 82, P 0 05), (r 0 89, P 0 01). At the same time, the location and geometry of the infarct shown by the NIRS topography were also confirmed by MRI and anatomical samples from the same rat. Conclusions The results suggest that NIRS can track changes in cerebral cortex blood flow caused by various factors in real time.