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目的:探讨喹啉衍生物PQ1联合顺铂对前列腺癌PC3细胞的增殖及细胞缝隙连接通讯的影响。方法:体外培养前列腺癌PC3细胞,对比空白对照组、10 mg/ml顺铂对照组及顺铂联合不同浓度(1、2、5、10、15μmol/L)喹啉衍生物PQ1各组PC3细胞增殖情况,通过RT-PCR法及Western印迹法检测各组间细胞缝隙连接相关蛋白Cx43 mRNA及蛋白表达情况。结果:喹啉衍生物PQ1浓度在1~10μmol/L联合顺铂能明显抑制PC3细胞的体外增殖,随浓度、时间的增加,抑制率相应升高。不同浓度联合药物处理PC3细胞后,Cx43 mRNA及蛋白表达也有不同程度升高。结论:喹啉衍生物可以通过上调前列腺癌PC3细胞间缝隙连接相关蛋白Cx43 mRNA及蛋白的表达水平来促进前列腺癌PC3细胞的细胞间缝隙连接功能,增强顺铂对前列腺癌PC3细胞的杀伤作用。
Objective: To investigate the effect of quinoline derivatives PQ1 combined with cisplatin on prostate cancer PC3 cell proliferation and cell gap junctional communication. Methods: Prostate cancer PC3 cells were cultured in vitro. PC3 cells were treated with different concentrations (1, 2, 5, 10 and 15 μmol / L) of quinoline derivatives PQ1 in 10 mg / ml cisplatin and 10 mg / Proliferation, Cx43 mRNA and protein expression of cell gap junction related proteins were detected by RT-PCR and Western blotting. Results: The concentration of PQ1 of quinoline derivatives combined with cisplatin (1 ~ 10μmol / L) significantly inhibited the proliferation of PC3 cells in vitro. With the increase of concentration and time, the inhibition rate of PCQ cells increased accordingly. Different concentrations of combined treatment of PC3 cells, Cx43 mRNA and protein expression also increased to varying degrees. CONCLUSION: Quinoline derivatives can promote the cell gap junctional function of prostate cancer PC3 cells and enhance the killing effect of cisplatin on prostate cancer PC3 cells by up-regulating the expression of Cx43 mRNA and protein in connective tissue of prostate cancer PC3 cells.