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用高辐射剂量照射的曼氏血吸虫尾蚴免疫大鼠,对攻击能产生很强的抗性。实验大鼠是一个新的有用的动物模型。本文对减毒日本血吸虫尾蚴免疫大鼠后产生的对攻击的抗性进行了研究。实验用8周龄Fisther F 344近交系大鼠,日本血吸虫为中国大陆株。供免疫的尾蚴经42mw/cm~2强度的紫外线照射45秒。大鼠麻醉后,腹部剃毛,皮肤接种经紫外线照射的尾蚴约1000条,4周后用500条正常尾蚴攻击,21天后门脉灌洗收集成虫估计免疫效果。在3次重复实验中,对攻击分别产生了52％,78％,和88％的抗性,均呈显著性差
Immunization of rats with Schistosoma mansoni cercariae exposed to high doses of radiation produced strong resistance to challenge. Experimental rat is a new and useful animal model. In this paper, we studied the resistance to challenge produced by attenuated S. japonicum cercariae immunized rats. Eight-week-old Fisther F 344 inbred strain rats and Schistosoma japonicum were Chinese mainland strains. Immune cercariae by 42mw / cm ~ 2 intensity of ultraviolet radiation for 45 seconds. After anesthesia in the abdomen, the abdomen was shaved and the skin was inoculated with about 1000 cercariae irradiated with ultraviolet rays. Four weeks later, the cells were challenged with 500 normal cercariae. After 21 days, the animals were dredged by portal vein to assess the immune effect. In 3 replicates, the challenge gave 52%, 78%, and 88% resistance, respectively, with significant differences