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免疫标记技术是一项微量免疫测定技术,它把标记物的高灵敏度和血清学反应的高度特异性结合起来,大大提高了方法的敏感性和特异性。目前,这项技术已广泛地应用于各个领域。常用的免疫标记技术有:放射免疫测定法(RIA)、酶联免疫吸附试验(ELISA)、免疫荧光技术等等。这些方法首先需要把已知的抗原或抗体固定在特定的固相上,然后加上含有相应抗体或抗原的溶液,因此,溶液中的抗体或抗原只能与包被于固相上的抗原或抗体的游离的结合部位发生反应,这就限制了抗原抗体作用的面
Immunolabeling is a micro-immunoassay technique that combines the high sensitivity of a label with the high specificity of a serological reaction to greatly increase the sensitivity and specificity of the method. At present, this technology has been widely used in various fields. Commonly used immunolabeling techniques: radioimmunoassay (RIA), enzyme-linked immunosorbent assay (ELISA), immunofluorescence technology and so on. These methods first require that the known antigen or antibody be immobilized on a particular solid phase and then add a solution containing the corresponding antibody or antigen so that the antibody or antigen in the solution can only bind to the antigen coated on the solid phase or The free binding site of the antibody reacts, which limits the surface of the antigen-antibody interaction