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目的:分析陕西荚蒾鲜叶挥发油的化学成分,并考察其体外抗菌活性。方法:采用水蒸气蒸馏法提取陕西荚蒾鲜叶挥发油,应用气质联用法分析挥发油的化学成分,应用峰面积归一化法确定各化学成分的相对质量分数,采用琼脂稀释法测定陕西荚蒾叶挥发油和环丙沙星对5种实验菌株的最小抑制浓度MIC值。结果:从陕西荚蒾叶挥发油中检出54种组分,鉴定出45种化合物。陕西荚蒾叶挥发油对金黄色葡萄球菌具有较强的抑菌活性,其MIC值为0.032 mg/mL。结论:陕西荚蒾叶挥发油主要成分为软脂酸(29.24%)、4-甲基-1-异丙基-双环[3.1.0]己酮(5.71%)、3-甲基-4-异丙苯酚(5.68%)和亚麻醇(4.84%),其挥发油对金黄色葡萄球菌具有较强的抑菌活性。
OBJECTIVE: To analyze the chemical constituents of the volatile oil from the fresh leaves of Viburnum chinense in Shaanxi Province and investigate its antimicrobial activity in vitro. Methods: Volatile oil from fresh leaves of Viburnum chinensis was extracted by steam distillation. The chemical constituents of volatile oil were analyzed by GC-MS method. The relative mass fraction of each chemical component was determined by peak area normalization method. Minimal Inhibitory Concentration (MIC) of Volatile Oil and Ciprofloxacin against 5 Experimental Strains. Results: Fifty-four components were detected from volatile oil of Viburnum chinense L., and 45 compounds were identified. Volatile oil from Viburnum fortunei has strong antibacterial activity against Staphylococcus aureus, with MIC value of 0.032 mg / mL. CONCLUSION: The main constituents of volatile oil in the leaves of Viburnum in Shaanxi are palmitate (29.24%), 4-methyl-1-isopropyl-bicyclo [3.1.0] hexanone Propofol (5.68%) and flax alcohol (4.84%), the volatile oil has a strong antibacterial activity against Staphylococcus aureus.