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以药用植物紫花地丁叶片和叶柄为外植体,经过不同灭菌处理等,进行紫花地丁组织培养与快繁技术的研究。结果表明,紫花地丁叶片用70%乙醇处理20 s、0.1%Hg Cl2处理6 min灭菌效果最佳,而叶柄的最佳灭菌条件为70%乙醇40 s+0.2%HgCl_24 min;叶片最适愈伤组织诱导培养基为2/3MS+2,4-D 0.2 mg/L+6-BA 0.8 mg/L,叶柄在MS+2,4-D 0.2 mg/L+6-BA 0.6 mg/L培养基上的诱导分化效果最好;MS+2,4-D 0.6 mg/L+6-BA 0.8 mg/L和MS+2,4-D 0.4 mg/L+6-BA 0.6 mg/L分别为叶片、叶柄诱导丛生芽和继代增殖的最佳培养基;最适生根培养基是1/2MS+2,4-D 0.4 mg/L+6-BA 0.2 mg/L+IBA 0.2 mg/L,生根率89%,移栽后,成活率高达95%。本研究成功建立了紫花地丁组织培养快繁体系,为其药用价值的进一步开发利用奠定了基础。
The medicinal plants Viola yedoensis leaves and petiole as explants, after different sterilization treatment, Viola yedo tissue culture and propagation technology. The results showed that the best sterilization conditions were as follows: 70% ethanol for 20 s, 0.1% Hg Cl2 for 6 min, the best sterilization condition for petiole was 70% ethanol for 40 s + 0.2% HgCl_24 min; The best medium for callus induction was 2 / 3MS + 2,4-D 0.2 mg / L + 6-BA 0.8 mg / L and petiole in MS + 2,4-D 0.2 mg / L + L medium with MS + 2,4-D 0.6 mg / L + 6-BA 0.8 mg / L and MS + 2,4-D 0.4 mg / L + 6-BA 0.6 mg / L The optimal culture medium was induced by leaf and petioles, respectively. The best medium for rooting was 1 / 2MS + 2,4-D 0.4 mg / L + 6-BA 0.2 mg / L + IBA 0.2 mg / L, the rooting rate of 89%, after transplanting, the survival rate of up to 95%. In this study, the tissue culture rapid propagation system of Viola yedoensis was successfully established, which laid the foundation for the further development and utilization of its medicinal value.