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用0～400Gy~(137)Csγ射线照射47个不同类型栽培大麦的休眠种子(含水量13％),研究其辐射敏感性。结果表明,幼苗生长抑制的剂量效应可用方程F(D)=1-(1—e~(α_1D)~N拟合,细胞核体积、根尖染色体畸变细胞率和过氧化物酶同工酶酶带的剂量效应可用线性回归方程Y=A+BX拟合。供试品种的辐射敏感性可分为5个类型,即极迟钝型、迟钝型、中间型、敏感型和极敏感型。试验还表明,核DNA含量和过氧化物酶同工酶酶谱与辐射损伤密切相关,其变化特点能反映出供试品种的辐射敏感性;大麦品种的辐射敏感性与DNA含量呈正相关;细胞核体积与核体积的D_(50)呈显著负相关,而与幼苗生长抑制的D_(50)无明显相关。
The dormant seeds (moisture content 13%) of 47 different types of cultivated barley were irradiated with 0 ~ 400Gy ~ (137) Csγ ray to study their radiation sensitivity. The results showed that the dose-effect of seedling growth inhibition could be fitted by equation F (D) = 1- (1-e ~ (α_1D) ~ N, cell volume, chromosome aberration rate and peroxidase isozymes The dose effects can be fitted by the linear regression equation Y = A + BX.The radiation sensitivity of the tested varieties can be divided into five types, namely very slow, slow, intermediate, sensitive and extremely sensitive.The experiment also shows , Nuclear DNA content and peroxidase isoenzyme zymogram closely related to radiation damage, and its variation can reflect the radiation sensitivity of the tested varieties; barley varieties of radiation sensitivity and DNA content was positively correlated; nuclear volume and nucleus The volume of D_ (50) showed a significant negative correlation with the seedling growth inhibition D_ (50) no significant correlation.