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圆盘电泳的基本原理,是丙烯酰胗和甲叉双丙烯酰胺在催化剂作用下聚合成三维网状结构的凝胶,具有分子筛、浓缩和电荷效应。用十二烷基磺酸钠与尿中蛋白质反应,形成带负电的十二烷基磺酸钠—蛋白质复合物,消除了蛋白质分子间原有的电荷差异。电泳时各蛋白质均向正极移动,通过聚丙悌酰胺的浓缩效应和分子筛作用,分子量小的
The basic principle of disk electrophoresis is that acrylamide and methylene bisacrylamide polymerize into a three-dimensional network structure gel under the action of a catalyst, which has molecular sieves, concentration and charge effects. Sodium dodecyl sulfate reacts with urinary proteins to form a negatively charged sodium dodecyl sulfate-protein complex that eliminates the original charge difference between protein molecules. Each electrophoretic protein moved toward the positive electrode, through the concentration effect of polypropylene amide and molecular sieve, the molecular weight of small