南疆温室番茄黄化曲叶病病毒种类的分子鉴定

• 作 者: 王喜刚 黄家风 都业娟
• 机 构: 石河子大学农学院新疆绿洲农业病虫害治理与植保资源利用自治区普通高校重点实验室,
• 出 处: 植物保护学报
• 发表日期: 2013年03期
• 关键词: 曲叶病毒 温室番茄 双生病毒 DNA 病毒种类 叶病 分子鉴定 分子检测 分离物 同源性比较

为明确南疆温室番茄黄化曲叶病的病毒种类,利用双生病毒的兼并引物通过PCR扩增,对采集的20个番茄病株进行了分子检测。从20个病株中均扩增到约500 bp的目标片段,对其中4株进行克隆和测序,其相互间序列同源性为97.1%～99.3%,与番茄黄化曲叶病毒(Tomato yellowleaf curl virus,TYLCV)的同源性较高,为98.6%～99.5%。随机选取莎车分离物KS2-5进行全基因组的克隆和测序,KS2-5 DNA全长为2781 nt(序列号:JQ807735),具有典型的双生病毒基因组特征,与TYLCV其它分离物同源性达到98.9%～99.5%,而与其它粉虱传双生病毒的序列同源性较低,为68.3%～75.5%,表明危害南疆温室番茄的病毒种类为番茄黄化曲叶病毒TYLCV。

In order to clarify the virus types of tomato yellow leaf curl disease in the greenhouse in South Xinjiang, 20 tomato tomato strains were molecularly detected by PCR amplification using the twin virus annex primers. The target fragment of about 500 bp was amplified from 20 diseased plants. Four of them were cloned and sequenced. Their sequence homology was 97.1% -99.3% with that of Tomato yellowleaf curl virus, TYLCV) had high homology of 98.6% -99.5%. The KS2-5 DNA was cloned and sequenced randomly. The full-length KS2-5 DNA was 2781 nt (SEQ ID NO: JQ807735) with typical characteristics of the geminivirus and its homology with other isolates of TYLCV 98.9% -99.5%, while the sequence identities with other whitefly transmitted geminiviruses were low, ranging from 68.3% to 75.5%, indicating TYLCV was the type of virus that harmed tomatoes in southern greenhouse.