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目的：探讨细胞凋亡和凋亡相关基因Bcl－2和Bax在正常前列腺（NP）和前列腺增生症（BPH）中的表述情况，明确细胞凋亡和Bcl-2，Bax在BPH形成中的作用。方法：采用DNA原位末端标记TUNEL法和免疫组织化学SABC法分别测定正常和增生的前列腺组织中细胞凋亡和凋亡相关基因Bcl-2，Bax的表达情况。结果：良性前列腺增生中存在着Bcl-2高表达情况，而细胞凋亡在良性前列腺增生中发生少见或根本未发生。两者与正常前列腺相比，Bcl-2表达显著高于正常前列腺组（P＜0.05），而细胞凋亡发生率显著低于正常前列腺组（P＜0．05）。Eax基因在正常前列腺、增生的前列腺中表达无明显差异（P＞0．05）。结论：细胞凋亡减少和Bcl2-的高表达参与BPH形成过程，Bcl-2表达增加，导致前列腺细胞凋亡减少可能是良性前列腺增生重要发病机制。
OBJECTIVE: To investigate the expression of apoptosis-related genes Bcl-2 and Bax in normal prostatic (NP) and benign prostatic hyperplasia (BPH) and to clarify the role of apoptosis and the expression of Bcl-2 and Bax in BPH . Methods: The expressions of Bcl-2 and Bax in apoptosis in normal and hyperplastic prostatic tissues were detected by TUNEL and immunohistochemical SABC method respectively. Results: There was a high expression of Bcl-2 in benign prostatic hyperplasia (BPH). Apoptosis occurred rarely or not in benign prostatic hyperplasia. The expression of Bcl-2 was significantly higher in normal prostate than in normal prostate (P <0.05), while the incidence of apoptosis was significantly lower than that in normal prostate (P <0.05). Eax gene expression in normal prostate, hyperplastic prostate showed no significant difference (P> 0.05). CONCLUSION: Apoptosis and high expression of Bcl2- are involved in the process of BPH formation. The increase of Bcl-2 expression leads to the decrease of apoptosis of prostatic cells, which may be an important pathogenesis of benign prostatic hyperplasia.