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采用新型栽培种(Neo-tuberosum,2n=48)为亲本,以IVP_(35)作授粉者,诱发孤雌生殖,获得了148份双单倍体无性系,从中筛选出能够产生FDR型2n配子无性系16份(纺锤体平行并融合类型11份,不联会突变型5份)。由于双单倍体孕性低或败育,以入选的16份双单倍体直接同S.tuberosum品系或品种杂交未能成功。同类型(A)入选的双单倍体无性系的系内或系间授粉,因配子体同型复等位基因作用产生的自交不亲和,也没能得到实生种子,而以产生2n配子的S.phureja品系的混合花粉授粉,在5个组合中获得25个浆果,共825粒杂交种子。
Newborn cultivars (Neo-tuberosum, 2n = 48) were used as parents and IVP_ (35) as pollinators to induce parthenogenesis. 148 double-haploid clones were obtained, and 2n gametes capable of producing FDR 16 clones (spindle parallel and fusion type 11, non-union mutant 5). Due to the low or aborted diploids, it was unsuccessful to directly cross the S. tuberosum strain or variety with the selected 16 doubled haploid species. Inbred or inter-pollination of selected two-haploid clones of the same type (A) did not result in self-fertilization due to the syngeneic allele of the gametophyte, Pollen pollen from the S. phureja strain was used to obtain 25 berries in 5 combinations for a total of 825 hybrid seeds.