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目的 观察单独或联合应用施万细胞和MK80 1对大鼠脊髓半横切后受损伤的背核神经元存活及其表达NOS的影响。 方法 应用细胞培养、免疫组织化学、核荧光标记、荧光逆行追踪和酶组织化学等技术。 结果 T11脊髓半横切后 15d ,生理盐水对照组的L1脊髓段损伤侧背核神经元的存活数量比未损伤侧减少 ,其中胞体面积 >4 0 0 μm2 的神经元明显减少 ,而且部分存活的神经元NOS活性增高 ,背核面积及神经元胞体面积缩小。施万细胞组、MK80 1组、施万细胞与MK80 1联合组的L1脊髓段损伤侧背核神经元的存活数量则比术后同期对照大鼠增多 ,胞体面积 >4 0 0 μm2 神经元的存活数量显著增多。施万细胞与MK80 1联合组作用明显。MK80 1组存活的神经元中NOS活性明显减弱 ,且能阻止背核及其神经元胞体的面积的缩小。 结论 施万细胞与MK80 1均可促进轴突被切断的背核神经元的存活 ,两者有协同作用 ,且MK80 1还能防止背核及其神经元胞体缩小
Objective To investigate the effects of Schwann cells and MK80 1, alone or in combination, on the survival and expression of NOS in injured spinal cord neurons after spinal hemisection in rats. Methods Cell culture, immunohistochemistry, nuclear fluorescent labeling, fluorescence retrograde tracing and enzyme histochemistry were used. Results Fifteen days after T11 spinal cord transection, the number of neurons in the dorsal radicular nucleus of L1 spinal cord lesioned in NS group decreased more than that in non-lesioned side, and neurons with cytoplasmic area> 400 μm2 were significantly reduced and partially survived NOS activity increased neurons, dorsal nucleus area and neuronal soma area reduced. Schwann cell group, MK80 1 group, Schwann cell and MK80 1 combined group of L1 spinal cord injury dorsal dorsal nucleus neurons survival compared with the same period after the control rats increased, cell body area> 400 μm 2 neurons The number of survivors increased significantly. Schwann cells and MK80 1 joint group obvious effect. NOS activity in MK80 1 survived neurons was significantly weakened, and the area of the dorsal nucleus and its neuronal soma could be prevented from decreasing. Conclusion Both Schwann cells and MK80 1 can promote the survival of axons excised dorsal nucleus. Both of them have a synergistic effect, and MK80 1 can prevent the dorsal nucleus and its neurons from shrinking