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异烟肼的含量测定有容量分析法、紫外、荧光、原子吸收和色谱等方法。英国药典(BP,1980年版)和美国药典(USP,1985年版)分别用溴量法和亚硝酸钠法,但这两种方法均不理想。本文采用异烟肼与6,7-二氯喹啉-5,8-二酮(DCQD)反应,生成有色化合物后进行分光光度法测定。该方法具有灵敏度、选择性和稳定性好等优点。标准溶液的制备:精密称取约50mg的标准异烟肼溶于水中,在100ml容量瓶中稀释至刻度,取这种溶液存一系列50ml容量瓶中制成20～250μg/ml的溶液,备用。样品溶液的制备:对纯品直接溶于水中配制成200μg/ml的溶液。对片剂、合成药的混
Isoniazid content determination of capacity analysis, UV, fluorescence, atomic absorption and chromatography and other methods. The British Pharmacopoeia (BP, 1980) and the United States Pharmacopeia (USP, 1985) use bromine and sodium nitrite, respectively, but neither method is satisfactory. In this paper, isoniazid and 6,7-dichloroquinoline-5,8-dione (DCQD) reaction, the formation of colored compounds spectrophotometric determination. The method has the advantages of sensitivity, selectivity and stability. Preparation of standard solution: accurately weighed about 50mg of standard isoniazid dissolved in water, diluted to the mark in a 100ml volumetric flask, take this solution stored in a series of 50ml volumetric flask made of 20 ~ 250μg / ml solution, spare . Preparation of sample solution: The pure product was directly dissolved in water to prepare a solution of 200 μg / ml. Mixing tablets, synthetic drugs