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[目的]观察先暴露于不同时间的汽油废气颗粒的大鼠再次受卵蛋白攻击后其迟发相反应的变化。[方法]雄性wistar大鼠随机分为6组,每组10只。A组给予生理盐水攻击;B组给予卵蛋白攻击。C、D、E、F4个组分别吸入汽油废气颗粒各1、2、3、4周后再用卵蛋白攻击。所有抗原攻击结束1周以后除A组用生理盐水激发30min以外其他组均用卵蛋白激发30min,24h后施行乙酰胆碱激发试验测定其气道阻力,然后比较每只大鼠乙酰胆碱激发后最终气道阻力和基础气道阻力的比值(简称气道阻力比值)。最后观察肺泡灌洗液中嗜酸性粒细胞百分比变化、肺组织中IL-5和IFN-γ的浓度以及肺组织的病理变化(气道壁厚度的变化)。[结果]气道阻力比值在B、C、D、E、F组均较A组增高,E、F组较B组增高,之间差异均有统计学意义(F=305.922,P﹤0.01)。气道阻力比值的增高和汽油废气颗粒吸入的时间有正相关性(r=0.938,P﹤0.01);肺泡灌洗液中嗜酸性粒细胞百分比经统计学分析,B组较A组增高,E、F组较B组增高,之间差异均有统计学意义(F=16.96,P﹤0.01);肺组织中IL-5在D、E、F组较A、B两组增高,差异有统计学意义(F=4.26,P﹤0.05);而肺组织中IFN-γ在D、E、F组较A、B两组降低,差异有统计学意义(F=12.098,P﹤0.05);大鼠气道壁厚度在B、C、D、E、F组较A组增高,E、F组较B组增高,之间差异均有统计学意义(F=106.233,P﹤0.01)。各组大鼠气道壁厚度与吸入时间呈正相关(F=106.233,r=0.894,P﹤0.01)。[结论]DEP吸入后的大鼠再次受卵蛋白攻击后其迟发相反应明显增强,其反应增强程度与DEP吸入时间呈正相关。
[Objective] To observe the change of delayed phase response of rats exposed to gasoline exhaust particles at different times after being attacked again by ovalbumin. [Methods] Male wistar rats were randomly divided into 6 groups of 10 rats. Group A was given saline challenge; Group B was challenged with egg protein. C, D, E, F 4 groups were inhaled gasoline exhaust particles 1,2,3,4 weeks after the egg protein attack. All rabbits in group A were stimulated with ovalbumin for 30 minutes after stimulated by saline for 1 week and those in group A stimulated by saline for 30 minutes. After 24 hours, the airway resistance was measured by acetylcholine provocation test, and the final airway resistance after each rat was stimulated by acetylcholine And basic airway resistance ratio (referred to as airway resistance ratio). Finally, the percentage of eosinophils in bronchoalveolar lavage fluid, the concentration of IL-5 and IFN-γ in lung tissue and the pathological changes of lung tissue (the change of airway wall thickness) were observed. [Result] The airway resistance ratio increased in group B, C, D, E and F than that in group A, and increased in group E and F compared with group B (F = 305.922, P <0.01) . The ratio of airway resistance was positively correlated with the inhalation time of gasoline exhaust particles (r = 0.938, P <0.01). The percentage of eosinophils in bronchoalveolar lavage fluid was higher than that in group A , F group increased compared with B group, the difference was statistically significant (F = 16.96, P <0.01); IL-5 in lung tissue in D, E, F group than A, B two groups increased, the difference was statistically (F = 4.26, P <0.05). However, the levels of IFN-γ in lung tissue were lower in groups D, E and F than those in groups A and B (F = 12.098, P <0.05) The thickness of rat airway wall increased in group B, C, D, E, F than in group A, and increased in group E, F compared with group B (F = 106.233, P <0.01). There was a positive correlation between airway wall thickness and inhalation time in each group (F = 106.233, r = 0.894, P <0.01). [Conclusion] After DEP inhalation, the delayed-phase response of the rats receiving OVP challenge was significantly enhanced. The extent of the response to DEP was positively correlated with the duration of DEP inhalation.