论文部分内容阅读
探讨了裂解酶组合、菌丝体培养基、菌丝体培养时间和酶作用时间对木耳原生质体释放的影响及菌丝体培养时间、再生培养基和渗透压稳定剂对木耳原生质体再生的影响；提出了木耳原生质体释放和再生的有效方法。
The effects of the combination of lyase, mycelium culture medium, mycelium culture time and enzyme action time on the release of protoplasts from Pleurotus ostreatus and the mycelium growth time, regeneration medium and osmolarity stabilizing agent on the regeneration of fungus protoplasts were studied The effective method for the release and regeneration of the fungus protoplasts was proposed.