载脂蛋白E基因多态性和Ⅱ型糖尿病肾病相关性的研究

• 作 者: 张莉 杨明功
• 出 处: 安徽医科大学学报
• 发表日期: 1999年02期
• 关键词: 糖尿病 非胰岛素依赖型 糖尿病肾病 载脂蛋白质类 多态性 限制性片段长度 聚合酶链反应

研究载脂蛋白Ｅ（ＡｐｏＥ）基因多态性与Ⅱ型糖尿病肾病的关系。方法以载脂蛋白Ｅ基因为候选基因，以相互之间无亲缘关系的５６例Ⅱ型糖尿病未合并肾病的患者、５８例Ⅱ型糖尿病肾病患者和７６例正常对照者为对象，应用聚合酶链反应（ＰＣＲ）扩增从白细胞中提取的载脂蛋白Ｅ基因ＤＮＡ片段，以限制性内切酶ＨｈａⅠ消化，经限制性片段长度多态性（ＲＦＬＰ）分析，区分６种ＡｐｏＥ的基因表型：Ｅ２／２、Ｅ２／３、Ｅ３／３、Ｅ３／４、Ｅ４／４。结果①３组研究对象的ε２、ε３、ε４等位基因分布频率分别为３６％、８３９％、１１５％，１４６％、７４１％、１１３％和５７％、８３１％、１１２％。Ⅱ型糖尿病肾病患者其ε２等位基因频率（１４６％）显著高于Ⅱ型糖尿病非肾病患者（３６％）（Ｐ＜０００５，χ２＝８３７）和正常对照者（５７％）（００２５＜Ｐ＜００５，χ２＝４２８），３组中ε３和ε４等位基因频率差异无显著性（Ｐ＞００５）。②通过比较Ⅱ型糖尿病患者不同的ＡｐｏＥ基因型和血脂的关系发现，ＡｐｏＥ基因多态性和血甘油三酯（ＴＧ）、总胆固醇（ＴＣ）和低密度脂蛋白胆固醇（ＬＤＬＣ）水平相关，携带ε２等位基因的个体ＴＧ水平? To study the relationship between apolipoprotein E (ApoE) gene polymorphism and type 2 diabetic nephropathy. Methods The apolipoprotein E gene was used as a candidate gene in 56 patients with type 2 diabetes without renal disease, 58 patients with type 2 diabetic nephropathy and 76 normal controls, which were unrelated to each other. Polymerase chain reaction The DNA fragment of apolipoprotein E gene extracted from leukocytes was amplified by PCR and digested with restriction endonuclease Hha I. The phenotypes of six ApoE genes were analyzed by restriction fragment length polymorphism (RFLP) E2 / 2, E2 / 3, E3 / 3, E3 / 4, E4 / 4. Results ① The frequencies of alleles ε2, ε3 and ε4 in group 3 were 36%, 839%, 115%, 146%, 741%, 113% and 55, respectively 7%, 831%, 112%. The frequency of ε2 allele (146%) in type Ⅱ diabetic nephropathy patients was significantly higher than that in type Ⅱ diabetic nephropathy patients (3.6%) (P0005, χ2 = 8.37) and normal controls 57%) (0025

0.05). ② By comparing the different ApoE genotypes and blood lipids in patients with type 2 diabetes, ApoE gene polymorphisms were found to be associated with triglyceride (TG), total cholesterol (TC), and low density lipoprotein cholesterol (LDL-C) levels , Carry ε2 allele individual TG level?