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本文建立了高效薄层色谱测定大鼠血浆中杀虫双和沙蚕毒素的方法。以甲醇处理血浆样品,取上清液于高效硅胶板上点样,用甲醇-乙酸乙酯(5:4)展开,喷以钙黄绿素-氯化钯溶液,以薄层色谱扫描仪定量测定斑点荧光强度。血浆中加入杀虫双和沙蚕毒素,回收率分别为97.3±2.6%(n=5,CV=2.65%)和94.9±3.9%(n=5,CV=3.92%),检测限杀虫双为11.9ng,沙蚕毒素为5ng。大鼠静脉注射杀虫双后,血浆中立即检出沙蚕毒素。根据开放型二室模型数学公式计算杀虫双及沙蚕毒素代谢动力学各参数值。经口灌胃后,各时相血浆样品中均未检出杀虫双原形。除个别一小时血浆样品中检出低浓度沙蚕毒素外,其他样品中也未检出沙蚕毒素。
In this paper, a method for the determination of insecticidal double and nereistoxin in rat plasma by high performance thin layer chromatography was established. The plasma sample was treated with methanol, the supernatant was spotted on a high efficiency silica gel plate, developed with methanol-ethyl acetate (5: 4), sprayed with a calcein-palladium chloride solution, and quantified by a TLC scanner The fluorescence intensity. The recoveries of pest and nereistoxin in plasma were 97.3 ± 2.6% (n = 5, CV = 2.65%) and 94.9 ± 3.9% (n = 5, CV = 3.92% 11.9ng, nereistoxin to 5ng. Rats intravenous insecticidal double immediately after the detection of nereistoxin in the plasma. The parameters of insecticidal double and nereistoxin metabolism were calculated according to the mathematical formula of open two-compartment model. Oral gavage, the plasma samples of each phase were not detected insecticidal double prototype. Nereis, toxins were not detected in any of the samples, except for the low nereistoxin detected in individual one-hour plasma samples.