论文部分内容阅读
作者用一次脾内免疫法制备抗人IgM单克隆抗体(McAb),并与常规免疫法进行比较.用乙醚麻醉小鼠,暴露脾脏,注入20μgIgM骨髓瘤蛋白,3天后取脾细胞与NSO细胞株融合.用固相放射结合试验(SPRBA)检测细胞培养上清液中抗IgM和IgG特异性抗体.脾内免疫法融合细胞共接种了192孔,其中23孔抗IgM阳性,阳性率为12%,虽然比常规法(31/192)稍低,但特异性较高.23株中只有1株和IgG有交叉反应,而用常规法得到的31株中,30株与IgG有交叉反应.
The authors used a spleen immunization method to prepare anti-human IgM monoclonal antibody (McAb) and compared with conventional immunization mice were anesthetized with ether to expose the spleen, injected with 20μgIgM myeloma protein, 3 days after spleen cells and NSO cell lines Fusion.The anti-IgM and IgG specific antibodies in cell culture supernatants were detected by SPRBA.The splenic immunofluorescent fusion cells were inoculated a total of 192 wells, of which 23 were anti-IgM positive, the positive rate was 12% , Although slightly less specific than the conventional method (31/192), had a higher specificity, and only one of 23 strains cross-reacted with IgG, while out of the 31 strains obtained by the conventional method, 30 were cross-reactive with IgG.