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目的:研究新城疫病毒(NDV)对鼻咽癌细胞的抑制作用及其作用机制。方法:采用化学发光法、流式细胞术及电子显微镜研究NDV分别作用于两株鼻咽癌细胞后其对细胞生长的抑制情况,以及NDV诱导鼻咽癌细胞发生凋亡的情况。结果:化学发光法的结果显示,在一定的浓度范围和时间范围内,NDV对鼻咽癌细胞的抑制作用有剂量效应比和时间效应比关系。流式细胞术结果显示,NDV使鼻咽癌细胞生长阻滞在G1期,并促进细胞凋亡。电子显微镜观察细胞凋亡超微结构的结果显示,NDV可诱导鼻咽癌细胞产生凋亡小体,发生凋亡。结论:NDV可以抑制鼻咽癌细胞增殖,其作用机制主要是影响细胞生长周期和诱导细胞发生凋亡。
Objective: To study the inhibitory effect of Newcastle disease virus (NDV) on nasopharyngeal carcinoma cells and its mechanism. Methods: Chemiluminescence, flow cytometry and electron microscopy were used to study the inhibitory effect of NDV on the growth of two nasopharyngeal carcinoma cells and the apoptosis of nasopharyngeal carcinoma cells induced by NDV. Results: The result of chemiluminescence showed that the inhibition effect of NDV on nasopharyngeal carcinoma cells in a certain range of concentration and time was dose-response and time-effect ratio. Flow cytometry showed that NDV blocked the growth of nasopharyngeal carcinoma cells in G1 phase and promoted cell apoptosis. The results of electron microscopy observation of ultrastructure of apoptosis showed that NDV could induce apoptosis of nasopharyngeal carcinoma cells. Conclusion: NDV can inhibit the proliferation of nasopharyngeal carcinoma cells. Its mechanism mainly affects the cell growth cycle and induces cell apoptosis.