论文部分内容阅读
96只Wistar大白鼠分为正常对照、肝硬变、激素负荷和免疫抑制4组模型,脾切除前后作病理组织学检查和测定多项免疫指标,比较术后抗感染能力。结果,脾切前后3组胸腺皮质、脾脏白髓都有不同程度萎缩,T淋巴细胞转化率、NK细胞活性和中性粒细胞吞噬细菌功能,皆明显低于正常对照组。脾切后正常对照组上述免疫指标下降,而后3组变化不大。脾切后抗感染能力,以免疫抑制组最差,激素负荷和肝硬变组次之。认为术前大量应用免疫抑制剂、皮质激素以及肝硬变所引起的免疫功能损害,是导致脾切后抗感染能力降低的主要因素,而切除有免疫障碍的脾脏可能是次要因素。
Ninety-six Wistar rats were divided into four groups: normal control, liver cirrhosis, hormone load and immunosuppression. The histopathological examination before and after splenectomy and determination of multiple immune parameters were performed to compare the postoperative anti-infective ability. Results: Before and after splenectomy, the thymus cortex and white pulp of the spleen had atrophy in different degrees. The T lymphocyte transformation rate, NK cell activity and neutrophil phagocytosis were all significantly lower than those in the normal control group. After the spleen cut, the above-mentioned immune index decreased in the normal control group, but there was no significant change in the latter three groups. The anti-infective ability after splenectomy was the worst in the immunosuppression group, followed by the hormone load and cirrhosis. The preoperative use of immunosuppressive agents, corticosteroids, and cirrhosis caused by immune dysfunction, is the main factor leading to reduced anti-infective ability after splenectomy, and the removal of immune disorders spleen may be a secondary factor.