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目的:建立并应用多重PCR技术检测疑似SWH1N1患者标本中病毒性病原体,了解此类患者的病毒感染情况,为传染性疾病的防控、临床诊疗等提供准确、及时的病原学依据。方法:采用ACPS引物多重PCR技术,对110份咽拭标本进行15种常见呼吸道病毒的检测。结果:110份标本中65个标本病毒核酸阳性,其中SWH1N1阳性者59份,甲型流感病毒(非SWH1N1亚型)阳性4份,RSV A/B阳性的4份,SWH1N1和RSV A/B的合并感染患者2例。结论:在SWH1N1流行季节过程中,人群中极少发生其他病毒性感染,该病毒的流行占有绝对优势。该病毒已经侵入的患者中极少发现病毒性混合感染。
OBJECTIVE: To establish and apply multiplex PCR to detect viral pathogens in suspected SWH1N1 patients, understand the virus infection in such patients, and provide accurate and timely etiological evidence for the prevention and control of infectious diseases, clinical diagnosis and treatment. Methods: The ACPS primer multiplex PCR was used to detect 15 common respiratory viruses in 110 pharyngeal swab specimens. Results: Of the 110 specimens, 65 samples were positive for nucleic acid, of which 59 were positive for SWH1N1, 4 were positive for influenza A (non-SWH1N1), 4 were positive for RSV A / B, and were positive for SWH1N1 and RSV A / B Infected patients in 2 cases. Conclusion: During the SWH1N1 epidemic season, other viral infections rarely occur in the population, and the prevalence of the virus has an absolute advantage. Viral mixed infections are rare in patients who have invaded the virus.