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目的:探讨辽宁地区CYP2D6基因C188T和ERCC1基因C8092A位点的单核苷酸多态性与肺癌易感性的关系。方法:采用病例-对照研究方法,选取肺癌患者和对照者各200例。应用KI法快速抽提人外周血基因组DNA,PCR-RFLP的方法检测CYP2D6基因C188T和ERCC1基因C8092A的单核苷酸多态性。结果:C188/C、C188/T和T188/T这3种基因型在病例组和对照组的分布频率分别为21.00%、49.50%、29.50%和18.50%、39.00%、42.50%,非T188/T基因型的个体发生肺癌的风险是T188/T基因型个体的1.78倍(95%CI=1.10-3.04),尤其在鳞癌中OR=2.57(95%CI=1.13-5.46)。按吸烟情况进行分层分析后发现不吸烟者及轻度吸烟者中携带非T188/T基因型的个体患肺癌的风险显著增高,OR值是3.14(95%CI=1.24-9.93)。非A8092/A基因型的个体发生肺癌的风险是A8092/A基因型个体的0.98(95%CI=0.59-2.04)。结论:T188/T基因型在不吸烟者和轻度吸烟者中可能作为保护因素而降低肺癌的易感性,ERCC1 C8092A多态性与肺癌易感性无相关性。
Objective: To investigate the relationship between single nucleotide polymorphisms of CYP2D6 C188T and ERCC1 C8092A loci in Liaoning Province and the susceptibility to lung cancer. Methods: A case-control study was conducted in 200 lung cancer patients and controls. The genomic DNA of human peripheral blood was rapidly extracted by KI method and the single nucleotide polymorphisms of CYP2D6 gene C8092A and C8092A of ERCC1 gene were detected by PCR-RFLP. Results: The frequencies of C188 / C, C188 / T and T188 / T genotypes were 21.00%, 49.50%, 29.50% and 18.50%, 39.00% and 42.50% The risk of lung cancer in individuals with T genotype was 1.78 times (95% CI = 1.10-3.04) in individuals with T188 / T genotype, especially in squamous cell carcinoma (OR = 2.57, 95% CI = 1.13-5.46). Stratification by smoking status found that individuals with non-T188 / T genotypes in non-smokers and mild smokers had a significantly higher risk of developing lung cancer, with a odds ratio of 3.14 (95% CI = 1.24-9.93). The risk of lung cancer in individuals not genotype A8092 / A is 0.98 (95% CI = 0.59-2.04) for individuals with the A8092 / A genotype. CONCLUSION: T188 / T genotype may be used as a protective factor in non-smokers and mild smokers to reduce the susceptibility to lung cancer. There is no correlation between ERCC1 C8092A polymorphism and lung cancer susceptibility.