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应用ＭＥＥ法对５９２株霍乱弧菌的１５个酶基因位点进行检测，分析和比较霍乱弧菌不同群体的群体遗传特征。结果：古典型菌株（ＣＶＣ）、埃尔托流行株（ＥＳＥＶＣ）和Ｏ１３９菌株（Ｏ１３９）的群体平均遗传多态值和平均杂合度都很低，ＨＣＶＣ＝０．１０６、ｈＣＶＣ＝１．５，ＨＥＳＥＶＣ＝０．０５６、ｈＥＳＥＶＣ＝２．３（Ｏ１３９包含在ＥＳＥＶＣ之中），被检测的基因位点多为非多态位点；而同为Ｏ１群菌的埃尔托非流行株（ＮＳＥＶＣ）的平均遗传多态值和平均杂合度要高得多，接近非Ｏ１群菌株（ＮＯ１）的群体遗传学指标，分别为ＨＮＳＥＶＣ＝０．４２５、ｈＮＳＥＶＣ＝５，ＨＮＯ１＝０．６２７、ｈＮＯ１＝６．５，被检测的基因位点几乎全是遗传多态位点。结果表明：ＥＳＥＶＣ和ＮＳＥＶＣ虽然都是Ｏ１群菌，但在群体遗传学特征上存在巨大差异；ＣＶＣ、ＥＳＥＶＣ和Ｏ１３９为遗传学性状稳定、群体遗传结构紧密的群体，而ＮＳＥＶＣ和ＮｏｎＯ１的群体遗传关系离散
The MEE method was used to detect 15 enzyme loci in 592 strains of V. cholerae to analyze and compare the population genetic characteristics of different groups of V. cholerae. Results: The average genetic polymorphism and average heterozygosity of the classical strains CVC, ESEVC and O139 were very low, HCVC = 0.106, hCVC = 1.5, HESEVC = 0.056, hESEVC = 2.3 (O139 is included in ESEVC), and most of the loci tested are non-polymorphic loci. Nonetheless, Elto non-epidemic strain (NSEVC) The average genetic polymorphism value and average heterozygosity were much higher than those of non-O1 group (NO1), which were HNSEVC = 0.425, hNSEVC = 5, HNO1 = 0.627, hNO1 = 6 .5, almost all genetic loci tested were genetic polymorphic loci. The results showed that although both ESEVC and NSEVC were O1 group bacteria, there were great differences in population genetic characteristics; CVC, ESEVC and O139 were stable genetic population and population heritability structure, but NSEVC and NonO1 population Genetic relationship is discrete