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对冠心病、心肌梗塞等疾病进行的病理、药理等研究中,常需要用心肌缺血和再灌注的动物模型。目前多采用特制气囊套管压迫狗冠状动脉的方法。但狗价昂,方法较复杂,难以推广采用。根据类似原理,我们建立了用兔制备心肌缺血和再灌注模型,兹介绍如下。方法健康成年家兔10只,体重2.5～3kg,雌雄不限。硫苯妥纳15mg 诱导麻醉,人工通气,随之用琥珀酰胆碱(0.12mg/kg·min)肌注,1%普鲁卡因局麻下手术。于左侧第4肋间开胸,在兔心左侧壁找出不与静脉伴行的冠脉左室支,于该动脉上部1/3～1/2处跨过动脉缝
Of coronary heart disease, myocardial infarction and other diseases in the pathology, pharmacology and other studies, often need to use myocardial ischemia and reperfusion animal model. Currently more use of special balloon catheter compression dog coronary artery method. But the dog price is expensive, the method is more complicated, it is difficult to popularize. According to a similar principle, we have established a rabbit model of myocardial ischemia and reperfusion, which are described below. Methods 10 healthy adult rabbits, weighing 2.5 ~ 3kg, male or female. Thiostatone 15 mg induced anesthesia, artificial ventilation, followed by intramuscular injection of succinylcholine (0.12 mg / kg · min), 1% procaine surgery under local anesthesia. Left intercostal space in the fourth intercostal thoracotomy in the left side of the rabbit heart to find the left ventricular branch of the coronary artery does not accompany the vein in the upper part of the artery 1/3 ~ 1/2 across the arterial