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从萤火虫尾中提取虫荧光素酶的方法已报道有多种,但对萤火虫捕捉后前处理方法报道不多,且未曾见到将各种前处理方法进行比较的报告。 作者将从浙江山区捕捉到的萤火虫按以下方法进行前处理:①将整虫干燥处理后,于常温下放入存有干燥剂的密闭容器中保存(干燥后常温保存)。②将整虫干燥处理后放入液氮中保存(干燥后液氮保存)。③将整虫放入液氮中保存数天后再进行冰冻干燥(液氮保存后再冻
A number of methods have been reported for the extraction of luciferase from firefly tails, but few have been reported for post-capture firefly pretreatment methods and no comparison of various pretreatment methods has been reported. The authors will be caught from the mountainous area of Zhejiang firefly pre-treatment by the following methods: ① the whole worm dried, stored at room temperature in a closed container containing desiccant (stored at room temperature after drying). ② The whole worm dried and placed in liquid nitrogen preservation (after drying liquid nitrogen preservation). ③ The whole insect into liquid nitrogen stored for a few days and then freeze-dried (liquid nitrogen and then frozen