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目的:研究人参皂苷Rg3对小鼠艾氏腹水癌的抑制作用及其相关机制。方法:建立昆明种小鼠艾氏腹水癌模型24小时开始用药,人参皂苷Rg3组采用灌胃给药20mg/kg.d,顺铂组0.5mg/kg.d和生理盐水组采用腹腔注射,每日1次,连续2周。治疗结束后24小时,处死各组半数小鼠测各项指标,剩余小鼠停止治疗并观察生存时间。结果:各用药组较生理盐水组在腹水量、腹水中瘤细胞数和瘤细胞存活率方面都下降。免疫组化结果显示:人参皂苷Rg3组的nm23和CD44的表达率分别为90%和70%,与生理盐水组比较均有统计学意义,MMP-2的表达率为80%,与生理盐水组比较无差异。人参皂苷Rg3组和顺铂组较生理盐水组小鼠寿命延长。结论:人参皂苷Rg3对小鼠恶性腹腔积液的形成及癌细胞有抑制作用,并可以延长其生存时间,其机制可能与调节nm23和CD44的表达有关。
Objective: To study the inhibitory effect of ginsenoside Rg3 on Ehrlich ascites carcinoma and its related mechanism. METHODS: A Kunming mouse model of Ehrlich ascites carcinoma was established for 24 hours. The ginsenoside Rg3 group was administered intragastrically with 20 mg/kg.d, the cisplatin group was 0.5 mg/kg.d and the saline group was injected intraperitoneally. Once a day for 2 weeks. Twenty-four hours after the end of the treatment, each group of half mice was sacrificed to measure each index, and the remaining mice were stopped for treatment and the survival time was observed. RESULTS: Compared with the normal saline group, each drug group had a decrease in the amount of ascites, the number of ascites tumor cells, and the survival rate of tumor cells. The results of immunohistochemistry showed that the expression rates of nm23 and CD44 in the ginsenoside Rg3 group were 90% and 70%, respectively. Compared with the saline group, the expression rates were both statistically significant. The expression rate of MMP-2 was 80% in the saline group. There is no difference. The ginsenoside Rg3 group and cisplatin group had longer life than the normal saline group. CONCLUSION: Ginsenoside Rg3 has inhibitory effects on the formation of malignant peritoneal effusion and cancer cells, and can prolong the survival time. Its mechanism may be related to the regulation of the expression of nm23 and CD44.