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1植物名称罗河石斛(Dendrobium lohohense Tanget Wang)。2材料类别带芽茎段。3培养条件(1)原球茎诱导培养基:MS+6-BA 1.0mg·L~(-1)(单位下同)+NAA 0.1+KT 0.5;(2)丛生芽增殖和分化培养基:MS+6-BA 2.0+NAA 0.1+5%香蕉提取物;(3)壮苗生根培养基:1/2MS+6-BA 0.1+IBA0.5+10%椰子汁。上述培养基中分别附加5 g·L~(-1)琼脂,pH 5.8～6.0。培养温度(25±1)℃;光照时间12 h·d~(-1),光照强度20～30μmol·m~(-2)·s~(-1)。
1 plant name Dendrobium lohohense Tanget Wang. 2 material categories with bud stem segments. 3 Culture conditions (1) Protocorm-induced medium: MS + 6-BA 1.0 mg · L -1 (unit same below) + NAA 0.1 + KT 0.5; (2) + 6-BA 2.0 + NAA 0.1 + 5% banana extract; (3) strong seedling rooting medium: 1/2 MS + 6-BA 0.1 + IBA 0.5 + 10% coconut milk. The above medium was added with 5 g · L -1 agar, pH 5.8 ~ 6.0. The culture temperature (25 ± 1) ℃, illumination time 12 h · d ~ (-1) and light intensity 20 ~ 30μmol · m -2 · s ~ (-1).