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建立用反相高效液相色谱法测定血浆中二乙三胺五醋酸(DTPA)浓度的方法,DTPA与内标物(烟酸)峰面积之比在DTPA 30～600μg/ml范围内呈直线关系(r=0.9994),回收率为97.4％,样品检出灵敏度为4μg/kg。家兔静脉注射及皮下注射DTPA的药—时曲线符合一室开放模型,按一级动力学消除。DTPA的药动学参数(x±s)皮下注射100mg/kg t_(1/2)。15±2.9min,t_(1/2)53.8±18min,Tmax 44±8min,Cmax274±115μg/ml,V.C/F 0.27±0.15L/kg。DTPA静脉注射50mg/kg t_(1/2) 29±3min,Vd 0.13±0.03L/kg,F 71.3％。
To establish a method for the determination of DTPA in plasma by reversed-phase high performance liquid chromatography (RP-HPLC), the ratio of DTPA to internal standard (nicotinic acid) peak area was linear in the range of 30 ~ 600 μg / ml DTPA (r = 0.9994), the recovery rate was 97.4%, the detection sensitivity of the sample was 4μg / kg. Rabbit intravenous injection and subcutaneous injection of DTPA drug - time curve in line with a room open model, according to a kinetic elimination. DTPA pharmacokinetic parameters (x ± s) subcutaneous injection of 100mg / kg t_ (1/2). 15 ± 2.9min, t 1/2 (1/2) 53.8 ± 18min, Tmax 44 ± 8min, Cmax 274 ± 115μg / ml, V.C / F 0.27 ± 0.15L / kg. DTPA was intravenously injected 50 mg / kg t_ (1/2) for 29 ± 3 min, Vd 0.13 ± 0.03 L / kg, F 71.3%.