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目的:研究硫酸钡海藻酸钠微球对兔VX2肝肿瘤的介入治疗作用。方法:新西兰兔24只,随机分为3组:正常对照组(A组,n=5)、肿瘤对照组(B组,n=10)和介入治疗组(C组,n=9)。介入治疗模拟人肝动脉插管方法。A、B、C组于介入术后7d行肝功能检查,B组、C组各5只动物于介入术后2周观察肿瘤质量、体积,并行病理学检查,包括常规H-E染色、CD34及VEGF免疫组化检测;B组5只、C组4只动物观察生存期。结果:介入治疗后7d,丙氨酸转氨酶(ALT)、天冬氨酸转氨酶(AST)水平B、C组均高于A组(P<0.01),但C组ALT、AST数值低于B组(P<0.01)。C组肿瘤质量(2.434±0.992)g,B组(4.696±1.246)g,差异具有统计学意义(P<0.01)。C组肿瘤体积(2.126±0.929)cm3,B组(3.962±1.101)cm3,差异具有统计学意义(P<0.01)。病理学检查结果显示C组癌细胞大片坏死,CD34染色大片坏死区无微血管,残存的癌组织内新生血管明显减少,VEGF表达仅见残存的癌细胞胞质呈弱阳性表达;而B组癌巢大,癌细胞丰富,CD34染色可见丰富的新生血管,癌细胞胞质可见丰富的VEGF表达。C组生存期明显延长。结论:硫酸钡海藻酸钠微球介入治疗兔VX2肝肿瘤安全可行;能明显抑制肿瘤生长,且对正常肝组织损伤小,能延长实验兔生存时间。
Objective: To investigate the interventional therapeutic effect of barium sulfate alginate microspheres on rabbit VX2 liver tumor. Methods: Twenty - four New Zealand rabbits were randomly divided into three groups: normal control group (n = 5), tumor control group (n = 10) and intervention group (n = 9). Interventional treatment of simulated human hepatic artery intubation. Liver function tests were performed in groups A, B and C at 7 days after intervention. Five animals in groups B and C were observed for tumor mass, volume and pathology 2 weeks after the intervention, including routine HE staining, CD34 and VEGF Immunohistochemical detection; B group 5, C group 4 animals observed survival. Results: The levels of ALT and AST in group B and C were higher than those in group A on the 7th day after intervention (P <0.01), but the values of ALT and AST in group C were lower than those in group B (P <0.01). The tumor mass in group C (2.434 ± 0.992) g and group B (4.696 ± 1.246) g, the difference was statistically significant (P <0.01). The tumor volume in group C was (2.126 ± 0.929) cm3 and in group B was 3.962 ± 1.101 cm3, the difference was statistically significant (P <0.01). The results of pathology showed that the large necrosis of the cancer cells in group C was necrotic, no microvessels were found in the necrotic area of CD34 staining, the number of neovascularization in the remaining cancerous tissue was significantly decreased, and the expression of VEGF in the remaining cancer cells was weakly positive. , Rich in cancer cells, rich in CD34 staining of neovascularization, abundant expression of VEGF in the cytoplasm of cancer cells. C group significantly prolonged survival. Conclusion: The intervention of barium sulfate alginate microspheres is safe and feasible for the treatment of rabbit VX2 liver tumor. It can significantly inhibit tumor growth and damage the normal liver tissue, prolong the survival time of experimental rabbits.